Journal: Frontiers in Veterinary Science

Article Title: Effects of Dietary Macleaya cordata Extract Containing Isoquinoline Alkaloids Supplementation as an Alternative to Antibiotics in the Diets on Growth Performance and Liver Health of Broiler Chickens

doi: 10.3389/fvets.2022.950174

Figure Lengend Snippet: Primer sequences used for quantitative real-time PCR.

Article Snippet: The mRNA expressions of SOD2 and GPX1 were significantly lower in the AGP group and MCE group than in the CON group ( p < 0.05), and no significant differences were observed in the mRNA expressions of Sirt1, Nrf2, HO-1, SOD1, CAT , and NQO1 among the groups ( p > 0.05).

Techniques:

Journal: Frontiers in Veterinary Science

Article Title: Effects of Dietary Macleaya cordata Extract Containing Isoquinoline Alkaloids Supplementation as an Alternative to Antibiotics in the Diets on Growth Performance and Liver Health of Broiler Chickens

doi: 10.3389/fvets.2022.950174

Figure Lengend Snippet: Effects of dietary MCE supplementation on expressions of antioxidation-related genes in livers of broiler chickens. (A) Sirtuin1 (Sirt1); (B) Nuclear factor erythroid 2-related factor 2 (Nrf2); (C) Heme-oxygenase 1 (HO-1); (D) Superoxide dismutase 1 SOD1; (E) Superoxide dismutase 2 (SOD2); (F) Catalase (CAT); (G) Glutathione peroxidase 1 (GPX1); (H) NAD(P)H: quinone oxidoreductase-1 (NQO1). CON, broiler chickens fed basal diet; AGP group, broiler chickens fed a basal diet supplemented with 50 mg/kg aureomycin; MCE, broiler chickens fed basal diet supplemented with 0.6 mg/kg Macleaya cordata extract (MCE) containing protopine and allocryptopine. Values are mean ± standard error ( n = 6). * p < 0.05, ** p < 0.01.

Article Snippet: The mRNA expressions of SOD2 and GPX1 were significantly lower in the AGP group and MCE group than in the CON group ( p < 0.05), and no significant differences were observed in the mRNA expressions of Sirt1, Nrf2, HO-1, SOD1, CAT , and NQO1 among the groups ( p > 0.05).

Techniques:

Journal: Acta biochimica et biophysica Sinica

Article Title: Vaccarin suppresses diabetic nephropathy through inhibiting the EGFR/ERK1/2 signaling pathway.

doi: 10.3724/abbs.2024141

Figure Lengend Snippet: Figure 3. VAC alleviated the renal inflammatory response and oxidative stress in T2DM mice (A) MDA contents in diabetic kidney tissues. n=6. (B) GSH-Px activity in diabetic kidney tissues. n=6. (C) Averaged fluorescence intensity of DHE fluorescence in diabetic kidney tissues. n=6. (D) Averaged fluorescence intensity of DCFH-DA fluorescence staining of diabetic kidney tissues. n=6. (E) DHE fluorescence staining or DCFH-DA fluorescence staining of diabetic kidney tissues. Scale bar: 100 μm. (F) F4/80 staining of diabetic kidney tissues. Scale bar: 50 μm, and the average fluorescence intensity of F4/80-expressing diabetic kidney tissues is shown. (G‒M) Representative blot images and quantitative analysis of phosphorylated NFκB P65, Nrf2, catalase, SOD3, SOD2 and SOD1. n=4. *P<0.05, **P<0.01, ***P<0.001 vs Ctrl. #P<0.05, ##P<0.01, ###P<0.001 vs DN.

Article Snippet: Primary antibodies against catalase, SOD1, SOD2 and SOD3 were procured from Boster Biological Technology (Wuhan, China).

Techniques: Activity Assay, Fluorescence, Staining, Expressing

Journal: Acta biochimica et biophysica Sinica

Article Title: Vaccarin suppresses diabetic nephropathy through inhibiting the EGFR/ERK1/2 signaling pathway.

doi: 10.3724/abbs.2024141

Figure Lengend Snippet: Figure 5. VAC alleviated the inflammatory response and oxidative stress in diabetic kidneys HK-2 cells were preincubated with 5 μM VAC for 12 h, followed by exposure to 35 mM HG for 48 h. (A–C) Relative mRNA levels of IL-1β, VCAM-1 and COX2. (D-F) DHE fluorescence staining or DCFH-DA fluorescence staining of HK-2 cells. Scale bar: 100 μm. (G–K) Relative mRNA levels of Nrf2, catalase, SOD3, SOD2 and SOD1. (L–R) Representative blot images and quantitative analysis of phosphorylated NFκB P65, Nrf2, catalase, SOD3, SOD2 and SOD1. *P<0.05, **P<0.01, ***P<0.001 vs NG. #P<0.05, ##P<0.01, ###P<0.001 vs HG. n=4.

Article Snippet: Primary antibodies against catalase, SOD1, SOD2 and SOD3 were procured from Boster Biological Technology (Wuhan, China).

Techniques: Fluorescence, Staining

Journal: Acta biochimica et biophysica Sinica

Article Title: Vaccarin suppresses diabetic nephropathy through inhibiting the EGFR/ERK1/2 signaling pathway.

doi: 10.3724/abbs.2024141

Figure Lengend Snippet: Figure 8. VAC ameliorated fibrosis, the inflammatory response and oxidative stress in HG-induced HK-2 cells exposed to the EGFR inhibitor AG1478 or the ERK inhibitor U0126 (A–D) Relative mRNA levels of collagen-1, TGF-β1, α-SMA and E-cadherin in HK-2 cells. (E–G) Relative mRNA levels of IL-1β, VCAM-1 and COX-2 in HK-2 cells. (H) Averaged fluorescence intensity of DHE fluorescence in HK-2 cells. (I) DHE staining was performed on HG-induced HK-2 cells. Scale bar: 100 μm. (J–P) Representative blot images and quantitative analysis of phosphorylated and total NFκB P65, Nrf2, catalase, SOD3, SOD2 and SOD1. *P<0.05, **P<0.01, ***P<0.001 vs NG. #P<0.05, ##P<0.01, ###P<0.001 vs HG. n=4.

Article Snippet: Primary antibodies against catalase, SOD1, SOD2 and SOD3 were procured from Boster Biological Technology (Wuhan, China).

Techniques: Fluorescence, Staining

Journal: Cell metabolism

Article Title: SELECTIVE PERSULFIDE DETECTION REVEALS EVOLUTIONARILY CONSERVED ANTI-AGING EFFECTS OF S -SULFHYDRATION

doi: 10.1016/j.cmet.2019.10.007

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: MnSOD , Creative BioMart , Cat# SOD2-1039H.

Techniques: Ab Array, Recombinant, Protease Inhibitor, Magnetic Beads, Sequencing, Mutagenesis, Plasmid Preparation, Software

Journal: Molecules

Article Title: Resveratrol Ameliorates Fibrosis in Rheumatoid Arthritis-Associated Interstitial Lung Disease via the Autophagy–Lysosome Pathway

doi: 10.3390/molecules27238475

Figure Lengend Snippet: Resveratrol treatment reversed the accumulation of P62 to enhance autophagy levels and attenuate fibrosis. ( A ) The mRNA level of LC3B was quantified. ( B , C ) The correlation between LC3B and FN1 and COL3A1 gene expression showed a stronger positive correlation. Spearman’s r and p values are indicated. ( D ) Levels of SOD2, HIF-1a, and LC3 in lung tissues were detected using Western blotting. ( E ) The immunoblotting data of LC3 II/I were quantified as described above. ( F , G ) The mRNA levels of P62 and BNIP3 were quantified. ( H ) The correlation between P62 and FN1 gene expression showed a stronger positive correlation. Spearman’s r and p values are indicated. ( I ) Levels of P62, p-P62(phospho-SQSTM1/p62(Ser349)), BNIP3, BECN1, and BCL2 in lung tissues were detected using Western blotting. ( J ) The relative intensity of P62 was quantified as described above using Western blotting. Values represent the mean ± SEM (n = 3–5). * p < 0.05 and ** p < 0.01 compared with the control group, and compared with the model group (CIA+BLM), # p < 0.05 and ## p < 0.01.

Article Snippet: Then, the membrane was sealed in 5% skim milk for 1.5 h. β-actin (Abcam, # ab8226), Collagen I (Arigo Biolaboratories, #ARG21965), HIF-1a (Proteintech, #20960-1-AP), LC3A/B (Cell signaling technology, #12741), P62 (Cell signaling technology, #16177S), Phospho-SQSTM1/p62(Ser349) (CST, #E7M1A), Bcl-2 (Abcam, #ab182858), BNIP3 (Santa Cruz Biotechnology, #sc-56167), BNIP3L (Proteintech, #12986-1-AP), BECN1 (Boster, #PB0014), and SOD2 (Boster, #BA4566) were used.

Techniques: Gene Expression, Western Blot, Control